NEW UPDATE!! We have made a new assembly, prediction and annotation of Pochonia chlamydosporia (isolate 123) genome. Now available in https://ncbi.nlm.nih.gov/genome/11596?genome_assembly_id=451721…
We have recently sequenced and functionally analyzed the genome of nematophagus fungus Pochonia chlamydosporia 123 isolate (Larriba et al., 2014) obtained from suppressive soils in Seville (SW Spain; Olivares-Bernabéu and Lopez-Llorca, 2002). The genome of the nematophagus fungus displays high similarities with that of the entomopathogenic fungus Metarhizium anisopliae, indicating a possible common origin of fungal invertebrate parasitism in both groups. The endophytic fungus behavior is also justified by the similarity of P. chlamydosporia genome with that of other fungal endophytes (non-nematophagous fungi). Finally, the expansion in the genome of P.chlamydosporia of hydrolytic enzyme families such as proteases and glycosidases (GH) again evidence the behavior of the multi-trophic versatile biocontrol agent.
The 8,574 contigs resulting from the Pochonia chlamydosporia genome assembly at the scaffold level were deposited in the EMBL/Genbank/DDBJ databases under accession numbers GCA_000411695.1 and AOSWGS00000000.
Protein sequences were predicted as in Larriba et al. 2014. These protein sequences are available for download.
Download protein fasta: P. chlamydosporia_proteins fasta file:
Transcripts sequences were predicted as in Larriba et al. 2014. These transcripts sequences are available for download.
Download transcripts fasta: P. chlamydosporia_transcripts fasta file:
Partial annotation of the genes (7000) from the P. chlamydosporia genome
PcB1_Fg_MevsPC_genes.fasta file contains the complete sequences of the genes of P. chlamydosporia genome. In this file we include gene sequences that after a bidirectional hit blast displayed 1, 2, 3 and even 4 hits with the transcripts file (the one already placed in fungalinteractions.org). We have selected the genes with a single hit (gene_hits.txt). For the rest of the genes we are working to obtain the consense sequences for all the genes in the genome. Besides we include a master key to relate the nomenclature of both files: the genes one PcB1_Fg_MevsPC_genes.fasta and the one of the transcripts (already present in fungalinteractions.org).
Please quote: Larriba et al. (2014) Fungal Genetics and Biology 65, pp. 69-80.